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gastrin 1  (Tocris)


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Bioz Manufacturer Symbol Tocris manufactures this product  
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    Structured Review

    Tocris gastrin 1
    Gastrin 1, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gastrin+1/Gastrin+I+(human)/pm41931852-45-43-45
    Average 96 stars, based on 165 article reviews
    gastrin 1 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: Pancreatic Cancer 3D Cell Line Organoids (CLOs) Maintain the Phenotypic Characteristics of Organoids and Accurately Reflect the Cellular Architecture and Heterogeneity In Vivo
    Article Snippet: The 3D organoids were maintained in Complete Human Feeding Media (CHFM), 50% v/v L-WRN conditioned media, 500 nM A83-01 (Sigma-Aldrich, SML0788, St. Louis, MO, USA), 100 ng/mL hFGF10 (Biolegend, 559308, San Diego, CA, USA), 50 ng/mL EGF (ThermoFisher, PHG0311, Waltham, MA, USA), 0.01 μM Gastrin 1 (Tocris, 3006, Bristol, UK), 1.25 mM N-acetylcysteine (R&D, 5619, Minneapolis, MN, USA), 10 mM Organoids 2022, 1 170 Nicotinamide (Sigma-Aldrich, N0636), 1× B-27 supplement (Life Technologies, 17504-044, Carlsbad, CA, USA), 10.5 μM Y-27632 (Sigma-Aldrich, Y0503) in DMEM-F12 (Merck, D642, Darmstadt, Germany) supplemented with 1 mM HEPES buffer (Merck, H3375) and 1× antibiotic-antimycotic (ThermoFisher, 15240062).

    Article Title: Fatty Acid Synthase (FASN) as a targeted therapeutic in acquired 5-fluorouracil resistance in pancreatic cancer.
    Article Snippet: The 3D organoids were maintained in Complete Media (CM): 50% v/v L-WRN conditioned media, 500 nM A83–01 (Sigma-Aldrich, SML0788, St. Louis, MO, USA), 100 ng/mL hFGF10 (Biolegend, 559308, San Diego, CA, USA), 50 ng/mL EGF (Thermo Fisher, PHG0311, Waltham, MA, USA), 0.01 μM Gastrin 1 (Tocris, 3006, Bristol, UK), 1.25 mM N-acetylcysteine (R&D, 5619, Minneapolis, MN, USA), 10 mM Nicotinamide (Sigma-Aldrich, N0636), 1 × B− 27 supplement (Life Technologies, 17504–044, Carlsbad, CA, USA), 10.5 μM Y− 27632 (Sigma-Aldrich, Y0503) in DMEM-F12 (Merck, D642, Darmstadt, Germany) supplemented with 1 mM HEPES buffer (Merck, H3375) and 1 × antibiotic-antimycotic (Thermo Fisher, 15240062).


    Centrifugation:

    Article Title: More powerful dysregulation of Helicobacter pylori East Asian-type CagA on intracellular signalings
    Article Snippet: In a nutshell, the stomach of approximately 1 cm 2 was firstly cut into small pieces and washed in cold Ca 2+ /Mg 2+ -free D-PBS (Procell, PB180329, China) until the supernatant was clear. .. Pieces were enzymatically digested by 1 mg/mL of collagenase and 0.5 mg/mL of hyaluronidase in 5 mL Advanced DMEM/F12 (Gibco, 12634010, USA) medium for 30 min. After repeated washing and centrifugation to remove enzymes, the gastric glandular cells were suspended and seeded into Matrigel (R&D system, 3533-005-02, USA) and maintained in medium containing: 100 ng/mL of Wnt3A (Peprotech, 315 − 20, USA), 100 ng/mL of Noggin (R&D system, 1967-NG, USA), 500 ng/mL of R-Spondin-1 (Peprotech, 315 − 32, USA), Glutamax-I (1×) (Gibco, 35050061, USA), 1% of Penicillin/streptomycin (Gibco, 15070063, USA),100 ng/mL of FGF-10 (R&D system, 6224-FG, USA), 50 ng/mL of EGF (R&D system, 2028-EG, USA), 1×B27 (Gibco, 17504044, USA), 10 nM of Gastrin-1 (Tocris Bioscience, 3006, UK), 1 mM of N-Acetylcysteine (Tocris Bioscience, 5619, UK) during the whole cultivation and 10 μM of Y-27,632 (Tocris Bioscience, 1254, UK) for only the first 2 days. ..

    Recombinant:

    Article Title: Dictionary of human intestinal organoid responses to secreted niche factors at single cell resolution
    Article Snippet: The tissue was then ready for plating in Matrigel (Corning) by resuspending in cold Matrigel and plating into 30uL Matrigel droplets, at a density of 30-50 crypts per droplet. .. Once Matrigel had solidified for 30 minutes at 37C, crypts were fed with organoid growth media : Advanced DMEM/F12 supplemented with 1X Pen-Strep, 10mM HEPES, 1X GlutaMAX, 1X B-27 Supplement (Thermo Fisher Scientific), 0.2nM Wnt Surrogate protein (ImmunoPrecise Antibodies), 5nM Gastrin-1 (Tocris), 1.25mM N-Acetyl-L-Cysteine (Sigma-Aldrich), 10mM Nicotinamide (Sigma-Aldrich), 100μg/mL Primocin (InvivoGen), 500nM A 83-01 (Tocris), 50ng/mL recombinant human EGF (R&D Systems), 100ng/mL recombinant human Noggin (R&D Systems), 250ng/mL recombinant human R-spondin 3 (Roche), 100ng/mL recombinant human IGF1 (BioLegend), 50ng/mL recombinant human FGF2 (PeproTech). .. After the initial organoid plating, cultures were maintained in Cultrex Basement Membrane ECM (70% Cultrex - PBS) (R&D Systems) domes.



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